custom affymetrix based dna microarray Search Results


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Thermo Fisher dna microarray analysis
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affymetrix 428 scanner  (MWG-Biotech ag)
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aza  (Thermo Fisher)
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microarray  (Incyte corporation)
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microarray  (Sequenom)
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dna microarrays  (Corning Life Sciences)
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stem cells transcription profiling dna microarray  (Thermo Fisher)
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Thermo Fisher stem cells transcription profiling dna microarray
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hybridization buffer  (Thermo Fisher)
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Thermo Fisher hybridization buffer
IECs up-regulate numerous ISGs in response to C parvum infection. Intestinal epithelium was harvested from ileum mucosa of neonatal piglets at the time of peak infection (days 3–5 after infection) (n = 8) and from age-matched uninfected controls (n = 4) for performance of gene expression analysis using microarrays. ( A ) Representative photomicrograph of ileum mucosa from an uninfected control and C parvum –infected piglet used for microarray analysis. H&E stain. Scale bar : 20 μm. ( B ) Villus height and crypt depth (μm) and the percentage of total villus IECs that were infected with C parvum in control (n = 4) and C parvum –infected (n = 8) piglets used for microarray analysis. Each data point represents the average of 5 measurements per piglet. Scale bars : means ± SD. ** P < .01, Student t test comparison between uninfected and C parvum –infected piglets. Heat map of significantly ( C ) up-regulated and ( D ) down-regulated genes in infected ( C parvum ) and control (Uninf) exfoliated ileum villus epithelial cells. Each control and infected biological replicate is represented. Gene IDs:fold change are listed to the right of the heat map. Known ISGs are highlighted in red. ( E ) qRT-PCR analysis of porcine ileum mucosal total cellular mRNA for the presence of ISG15 mRNA. Samples were obtained from piglets used for microarray analysis at peak C parvum infection (days 3–5, n = 5) and age-matched controls (n = 5). For each sample, the Ct value for ISG15 was normalized to expression of the housekeeping gene cyclophilin (ΔCt). Fold-change differences between each sample were compared with a representative uninfected sample using the 2 -ΔΔCt method. Scale bars : means ± SD. ** P < .01, Student t test comparison between uninfected and C parvum –infected piglets. ( F ) Fluorescence in situ <t>hybridization</t> showing ISG15 mRNA (red fluorescence) in villus epithelial cells of ileum mucosa from piglets at peak C parvum infection and absent in villus epithelium of control piglets. Photomicrograph representative of results of 2 independent experiments. Scale bar : 50 μm.
Hybridization Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluidics station  (Thermo Fisher)
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Thermo Fisher fluidics station
IECs up-regulate numerous ISGs in response to C parvum infection. Intestinal epithelium was harvested from ileum mucosa of neonatal piglets at the time of peak infection (days 3–5 after infection) (n = 8) and from age-matched uninfected controls (n = 4) for performance of gene expression analysis using microarrays. ( A ) Representative photomicrograph of ileum mucosa from an uninfected control and C parvum –infected piglet used for microarray analysis. H&E stain. Scale bar : 20 μm. ( B ) Villus height and crypt depth (μm) and the percentage of total villus IECs that were infected with C parvum in control (n = 4) and C parvum –infected (n = 8) piglets used for microarray analysis. Each data point represents the average of 5 measurements per piglet. Scale bars : means ± SD. ** P < .01, Student t test comparison between uninfected and C parvum –infected piglets. Heat map of significantly ( C ) up-regulated and ( D ) down-regulated genes in infected ( C parvum ) and control (Uninf) exfoliated ileum villus epithelial cells. Each control and infected biological replicate is represented. Gene IDs:fold change are listed to the right of the heat map. Known ISGs are highlighted in red. ( E ) qRT-PCR analysis of porcine ileum mucosal total cellular mRNA for the presence of ISG15 mRNA. Samples were obtained from piglets used for microarray analysis at peak C parvum infection (days 3–5, n = 5) and age-matched controls (n = 5). For each sample, the Ct value for ISG15 was normalized to expression of the housekeeping gene cyclophilin (ΔCt). Fold-change differences between each sample were compared with a representative uninfected sample using the 2 -ΔΔCt method. Scale bars : means ± SD. ** P < .01, Student t test comparison between uninfected and C parvum –infected piglets. ( F ) Fluorescence in situ <t>hybridization</t> showing ISG15 mRNA (red fluorescence) in villus epithelial cells of ileum mucosa from piglets at peak C parvum infection and absent in villus epithelium of control piglets. Photomicrograph representative of results of 2 independent experiments. Scale bar : 50 μm.
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apo ar  (Thermo Fisher)
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Thermo Fisher apo ar
IECs up-regulate numerous ISGs in response to C parvum infection. Intestinal epithelium was harvested from ileum mucosa of neonatal piglets at the time of peak infection (days 3–5 after infection) (n = 8) and from age-matched uninfected controls (n = 4) for performance of gene expression analysis using microarrays. ( A ) Representative photomicrograph of ileum mucosa from an uninfected control and C parvum –infected piglet used for microarray analysis. H&E stain. Scale bar : 20 μm. ( B ) Villus height and crypt depth (μm) and the percentage of total villus IECs that were infected with C parvum in control (n = 4) and C parvum –infected (n = 8) piglets used for microarray analysis. Each data point represents the average of 5 measurements per piglet. Scale bars : means ± SD. ** P < .01, Student t test comparison between uninfected and C parvum –infected piglets. Heat map of significantly ( C ) up-regulated and ( D ) down-regulated genes in infected ( C parvum ) and control (Uninf) exfoliated ileum villus epithelial cells. Each control and infected biological replicate is represented. Gene IDs:fold change are listed to the right of the heat map. Known ISGs are highlighted in red. ( E ) qRT-PCR analysis of porcine ileum mucosal total cellular mRNA for the presence of ISG15 mRNA. Samples were obtained from piglets used for microarray analysis at peak C parvum infection (days 3–5, n = 5) and age-matched controls (n = 5). For each sample, the Ct value for ISG15 was normalized to expression of the housekeeping gene cyclophilin (ΔCt). Fold-change differences between each sample were compared with a representative uninfected sample using the 2 -ΔΔCt method. Scale bars : means ± SD. ** P < .01, Student t test comparison between uninfected and C parvum –infected piglets. ( F ) Fluorescence in situ <t>hybridization</t> showing ISG15 mRNA (red fluorescence) in villus epithelial cells of ileum mucosa from piglets at peak C parvum infection and absent in villus epithelium of control piglets. Photomicrograph representative of results of 2 independent experiments. Scale bar : 50 μm.
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Image Search Results


IECs up-regulate numerous ISGs in response to C parvum infection. Intestinal epithelium was harvested from ileum mucosa of neonatal piglets at the time of peak infection (days 3–5 after infection) (n = 8) and from age-matched uninfected controls (n = 4) for performance of gene expression analysis using microarrays. ( A ) Representative photomicrograph of ileum mucosa from an uninfected control and C parvum –infected piglet used for microarray analysis. H&E stain. Scale bar : 20 μm. ( B ) Villus height and crypt depth (μm) and the percentage of total villus IECs that were infected with C parvum in control (n = 4) and C parvum –infected (n = 8) piglets used for microarray analysis. Each data point represents the average of 5 measurements per piglet. Scale bars : means ± SD. ** P < .01, Student t test comparison between uninfected and C parvum –infected piglets. Heat map of significantly ( C ) up-regulated and ( D ) down-regulated genes in infected ( C parvum ) and control (Uninf) exfoliated ileum villus epithelial cells. Each control and infected biological replicate is represented. Gene IDs:fold change are listed to the right of the heat map. Known ISGs are highlighted in red. ( E ) qRT-PCR analysis of porcine ileum mucosal total cellular mRNA for the presence of ISG15 mRNA. Samples were obtained from piglets used for microarray analysis at peak C parvum infection (days 3–5, n = 5) and age-matched controls (n = 5). For each sample, the Ct value for ISG15 was normalized to expression of the housekeeping gene cyclophilin (ΔCt). Fold-change differences between each sample were compared with a representative uninfected sample using the 2 -ΔΔCt method. Scale bars : means ± SD. ** P < .01, Student t test comparison between uninfected and C parvum –infected piglets. ( F ) Fluorescence in situ hybridization showing ISG15 mRNA (red fluorescence) in villus epithelial cells of ileum mucosa from piglets at peak C parvum infection and absent in villus epithelium of control piglets. Photomicrograph representative of results of 2 independent experiments. Scale bar : 50 μm.

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Interferon-λ3 Promotes Epithelial Defense and Barrier Function Against Cryptosporidium parvum Infection

doi: 10.1016/j.jcmgh.2019.02.007

Figure Lengend Snippet: IECs up-regulate numerous ISGs in response to C parvum infection. Intestinal epithelium was harvested from ileum mucosa of neonatal piglets at the time of peak infection (days 3–5 after infection) (n = 8) and from age-matched uninfected controls (n = 4) for performance of gene expression analysis using microarrays. ( A ) Representative photomicrograph of ileum mucosa from an uninfected control and C parvum –infected piglet used for microarray analysis. H&E stain. Scale bar : 20 μm. ( B ) Villus height and crypt depth (μm) and the percentage of total villus IECs that were infected with C parvum in control (n = 4) and C parvum –infected (n = 8) piglets used for microarray analysis. Each data point represents the average of 5 measurements per piglet. Scale bars : means ± SD. ** P < .01, Student t test comparison between uninfected and C parvum –infected piglets. Heat map of significantly ( C ) up-regulated and ( D ) down-regulated genes in infected ( C parvum ) and control (Uninf) exfoliated ileum villus epithelial cells. Each control and infected biological replicate is represented. Gene IDs:fold change are listed to the right of the heat map. Known ISGs are highlighted in red. ( E ) qRT-PCR analysis of porcine ileum mucosal total cellular mRNA for the presence of ISG15 mRNA. Samples were obtained from piglets used for microarray analysis at peak C parvum infection (days 3–5, n = 5) and age-matched controls (n = 5). For each sample, the Ct value for ISG15 was normalized to expression of the housekeeping gene cyclophilin (ΔCt). Fold-change differences between each sample were compared with a representative uninfected sample using the 2 -ΔΔCt method. Scale bars : means ± SD. ** P < .01, Student t test comparison between uninfected and C parvum –infected piglets. ( F ) Fluorescence in situ hybridization showing ISG15 mRNA (red fluorescence) in villus epithelial cells of ileum mucosa from piglets at peak C parvum infection and absent in villus epithelium of control piglets. Photomicrograph representative of results of 2 independent experiments. Scale bar : 50 μm.

Article Snippet: The fragmented complementary RNA was diluted in hybridization buffer (2 N -morpholino-ethanesulfonic acid, NaCl, EDTA, Tween 20, herring sperm DNA, acetylated bovine serum albumin) containing biotin-labeled OligoB2 and Eukaryotic Hybridization Controls (Affymetrix).

Techniques: Infection, Gene Expression, Control, Microarray, Staining, Comparison, Quantitative RT-PCR, Expressing, Fluorescence, In Situ Hybridization